Comparison of CPF3 and CPF4 expression in male pharate adults (P24). Probes for both genes hybridize equally to the limbs (A, B). However, only CPF4 hybridizes to abdominal sclerites (D), a location not seen with CPF3 (C). (E) RT-qPCR of CPF3 and CPF4 in pharate adult anterior (head and thorax) and posterior (abdomen) at P24. Standard errors are shown. ism, intersegmental membrane; scl, sclerites; ms, muscle.