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Figure 1 | Parasites & Vectors

Figure 1

From: Estimating trematode prevalence in snail hosts using a single-step duplex PCR: how badly does cercarial shedding underestimate infection rates?

Figure 1

Single-round duplex PCR detection method for single and double trematode infections in snails. Agarose gels showing amplicons produced by single-round duplex PCR reactions for single and double trematode infections in snails, based on ITS rDNA sequences: (A) PCR products of infected G. adansonii tissues (Ebro samples). From left to right, L1 shows a 1500 bp DNA ladder, lane 1 M. obovata (537 bp), lane 2 C. labracis (301 bp), lane 3 artificially mixed infection, lane 4 negative control. (B) PCR products of infected Z. subcarinatus tissues (Otago samples). From left to right, L2 shows a 1000 bp DNA ladder, lane 5 M. novaezealandensis (214 bp), lane 6 Philophthalmus sp. (352 bp), lane 7 artificially mixed infection, lane 8 negative control.

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