Laboratory assessment of sensitive molecular tools for detection of low levels of Echinococcus multilocularis-eggs in fox (Vulpes vulpes) faeces

Table 1 Results of the molecular analysis of E. multilocularis eggs-only samples using two extraction methods

A summary of the results of the molecular analysis of pure E. multilocularis egg samples using two different DNA extraction methods (egg sieving and DNA-fishing) and subsequent analysis with up to four different PCR methods (Taq-PCR, EVA-PCR, mPCR and CO1rtPCR. Three replicates were run for all methods except for EVA-PCR which was run only once due to limited material. A sample was categorised as being positive if either of the replicates produced a positive result. Egg numbers added to each sample are available in Additional file 1: Table S1.

ISSN: 1756-3305