Table 1 Primers used in this study
Primer | Sequence (5′ → 3′) |
|---|---|
Generation of recombinant Cs Mb protein | |
Cs Mb-F | AGAGGATCCATGGCACCCCTATCAA |
Cs Mb-R | CGTCTCGAGTTAGCCAAGAAAGCCG |
Preparation of mutant CsMb proteins | |
Cs Mb-Y34A-F | GAATTTGGAAAAGCAGTCGCCATGGCTCTGTTCTCAG |
Cs Mb-Y34A-R | CTGAGAACAGAGCCATGGCGACTGCTTTTCCAAATTC |
Cs Mb-Y68A-F | GAGGGGATTAAGTACGCCGGCCAGACCTTTGC |
Cs Mb-Y68A-R | GCAAAGGTCTGGCCGGCGTACTTAATCCCCTC |
Detection of CsMb transcripts via RT-PCR | |
Cs Mb-RT-F | GCTGAACCCGTTGGTGAGTA |
Cs Mb-RT-R | TCACTGGTAGAGATGAAAGGGC |
Cs β-actin -RT-F | ACCGTGAGAAGATGACGCAGA |
Cs β-actin -RT-R | GCCAAGTCCAAACGAAGAATT |
Mouse specific primers | |
iNOS-F | GCAATATAGGCTCATCCAG |
iNOS-R | AACTCGCTCCAAGATTCC |
Sod1-F | ATTACAGGATTAACTGAAGG |
Sod1-R | CAATGATGGAATGCTCTC |
Sod2-F | ACAAACCTGAGCCCTAAG |
Sod2-R | CTCCCAGTTGATTACATTCC |
β-actin -F | AACCGCGAGAAGATGACCCAGATCATGTTT |
β-actin -R | AGCAGCCGTGGCCATCTCTTGCTCGAAGTC |