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Table 2 Rate constants of reactions between different mutants and H 2 O 2

From: Molecular characterization of Clonorchis sinensis secretory myoglobin: Delineating its role in anti-oxidative survival

Cs Mb

Guaiacol

ABTS

Vmax

Km

Vmax

Km

Wild-type

46

81

167

66

Y34A

N.D. *

N.D. *

N.D. *

N.D. *

Y68A

7.1

590

32

83

Y34A/Y68A

N.D. *

N.D. *

N.D. *

N.D. *

  1. Reactions were performed with H2O2 (~20 μM) and oxygenated rCsMb solutions at 20 °C and pH 7.0. The units are as follows: Vmax, nmol of product/nmol of rCsMb /min; and Km, mM.
  2. *Reaction rates were too slow to measure.
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Parasites & Vectors

ISSN: 1756-3305

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