Figure 3

Effects of peptidase inhibitors on the proteolytic profiles of the A. albopictus midgut and quantitative in-solution assays for the proteolytic activities. (A) Samples were pre-incubated for 30 min in the presence of 1 mM PMSF, 100 μM TLCK, 100 μM TPCK and 10 μM E-64. The proteolytic activities were detected after incubating the gels for 12 h at 37°C in Tris–HCl buffer, pH 7.5. The control samples were processed in the same way but in the absence of inhibitors. The numbers on the left indicate the apparent molecular masses of the peptidases (kDa). (B) The in-solution assays were performed using the fluorogenic substrate Z-Phe-Arg-AMC in the absence (control) or presence of 5 mM PMSF, 100 μM TLCK, 100 μM TPCK and 20 μM E-64 in 100 mM Tris–HCl buffer, pH 7.5.