Establishment of the LAMP assay. (A: Left Panel) Amplification of LAMP of f-DNA (63°C for 40 min) and restriction digestion of LAMP products. Lanes: M, DNA marker; 1, LAMP products of f-DNA; 2, water control; 3, Eco RI digestion of LAMP products. (B: Right-top Panel) Specificity of LAMP assay for E. multilocularis DNA. Lanes: M, DNA marker; 1 and 2, g-DNA from E. multilocularis larvae and f-DNA; 3 to 9, g-DNA of E. granulosus (G1 strain), E. shiquicus, T hydatigena, T. pisiformis, T. taeniaeformis, T. multiceps, D. caninum parasites; 10, g-DNA from dog intestinal tissues; 11, no f-DNA; 12, water control. (C: Right-bottom Panel) Sensitivity of LAMP method using different numbers of E. multilocularis eggs per gram of feces. Lanes: M, DNA marker; 1 to 5, one to five eggs; 6, ten eggs; 7, fifteen eggs; 8, twenty eggs; 9, negative control.