Expression of recombinant gametocyte protein EnGAM22. (A) Analysis of the expressed protein by SDS-PAGE. Protein marker (lane 1), the recombinant bacterial BL21 containing recombinant expression vector induced by 1 mM IPTG (lane 2), the recombinant bacterial BL21 not induced by IPTG (lane3), the bacterial containing the wild type vector induced by IPTG (lane 4) and the bacterial BL21 induced by IPTG (lane 5). (B) The solubility analysis of recombinant protein. Protein marker (lane 1), supernatant of bacterial sonicates (lane 2) and sediments of bacterial sonicates (lane 3).