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Figure 3 | Parasites & Vectors

Figure 3

From: Cloning and characterization of an Eimeria necatrix gene encoding a gametocyte protein and associated with oocyst wall formation

Figure 3

Western blotting analysis of rEnGAM22. The expressed protein was separated by SDS-PAGE and transferred to NC membranes, at the same time the wild type vector transferred bacterial was used as control. In (A) and (B), anti-6 × His tag monoclonal antibody (A) and mouse anti-GAM22 polyclonal antibody (B) were used as first antibody, individually. Protein marker (lane 1), the control (lane 2) and the recombinant protein (lane 3). (C) The convalescent sera from chickens were used as first antibody, anti-E. necatrix (lane 1), anti-E. tenella (lane 2) and anti-E. maxima (lane 3). (D) The gametocyte extracts were detected by the mouse anti-rEnGAM22 antibody. Gametocyte extracts (lane 1), protein marker (lane 2), and purified rEnGAM22 (lane 3).

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Parasites & Vectors

ISSN: 1756-3305

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