Table 1 Primers used in this study
Purpose | Species | Gene | Sequence (5’ to 3’) | Product size (bp) | |
|---|---|---|---|---|---|
Cloning | Leptotrombidium pallidum Leptotrombidium scutellare | EF1α | Fa | TATTGATGCTCCTGGTCACAG | 850 |
Rb | GAATTTGCAAGCAATGTGAGC | ||||
RpS3 | F | GARGAYGGNTAYTCHGGB | 438 | ||
R | CATRAYYTTNACYTTRATDCC | ||||
Standard | Drosophila melanogaster | RpS3 | F | CACGTTTCCGATTCGACGTC | 925 |
R | CACAACGGACACATTGTCGG | ||||
Apis mellifera | RpS3 | F | CTCGTGAACTGTCAGAAGATG | 794 | |
R | CTGCAAGTGGTATTGGTTGTG | ||||
Tetranychus urticae | RpS3 | F | TAGACGAATTCCTTCGTCGAG | 553 | |
R | AGAAACGTTGTCAGGTAATGGT | ||||
Leptotrombidium pallidum Leptotrombidium scutellare | EF1α | F | TTGATGCTCCTGGTCACAGA | 842 | |
R | GCAAGCAATGTGAGCAGTGT | ||||
RpS3 | F | ATCATCATTCTCGCCACGCG | 447 | ||
R | CTTGTCGCAGTAACACATGCC | ||||
qPCR | Drosophila melanogaster | RpS3 | F | CATTGAGTTGTACGCCGAGA | 127 |
R | ATGTAGCGGAGCACACCATAG | ||||
Apis mellifera | RpS3 | F | GTTGTGAAGTGGTTGTTAGTG | 132 | |
R | GAAGTACATGACGAGTTGCAG | ||||
Tetranychus urticae | RpS3 | F | ATGTGAAGTTGTCGTTTCCGG | 96 | |
R | TTACAAGGGTCACCAGCGTG | ||||
Leptotrombidium pallidum | EF1α | F | GTTAAGGAATTGCGCAGAGG | 123 | |
R | GTAACCGTTGGCGATTTGTC | ||||
RpS3 | F | TCTGACAGAGGCTTGTGTGC | 127 | ||
R | AGCCTTTCGCTCCAGATTCC | ||||
Leptotrombidium scutellare | EF1α | F | CCGGAGATTGGAACGAAAGG | 120 | |
R | TGGACACAACTGAACCACCC | ||||
RpS3 | F | GCACAATGCGAGTCTCTTCG | 111 | ||
R | GACTTCGCAACCTTTCGCTC | ||||