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Figure 2 | Parasites & Vectors

Figure 2

From: Engineered single nucleotide polymorphisms in the mosquito MEK docking site alter Plasmodium berghei development in Anopheles gambiae

Figure 2

SNPs within MEK D-site and catalytic domain alter Anopheles gambiae ERK phosphorylation in vitro . A, ERK phosphorylation (pERK) levels in Sua5B cells transfected with pMEK1-5 were first normalized to total ERK, and then to pERK levels in control cells transfected with the unaltered allele (wtMEK; set here as 1.0). The introduction of S243E and S243E/S247D mutations in pMEK1 and pMEK2, respectively, increased pERK levels, although not significantly, in transfected cells relative to control cells. However, pERK levels in cells transfected with catalytically active MEK K3M/K6M (pMEK5) were significantly lower than levels observed in cells transfected with catalytically active pMEK2 (ANOVA, Bonferroni’s test for pairwise comparisons, P < 0.05). Data are represented as means ± SEMs (N = 5). B, Representative western blots showing phosphorylated ERK, total ERK, and FLAG in Sua5B cells transfected with plasmids as in A. Detection of FLAG confirmed MEK overexpression.

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