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Figure 4 | Parasites & Vectors

Figure 4

From: Engineered single nucleotide polymorphisms in the mosquito MEK docking site alter Plasmodium berghei development in Anopheles gambiae

Figure 4

Midgut-specific overexpression of exogenous variant MEK alleles was confirmed by qRT-PCR. Total RNA was isolated from the midguts or carcasses of non-transformed (NT) female mosquitoes and female mosquitoes transformed with either pMEK2 or pMEK5 for qRT-PCR with probes that distinguished between exogenous variant and endogenous MEK alleles. Relative to levels of endogenous MEK mRNA in NT females, expression levels of exogenous variant MEK allele were four- or three-fold higher (P < 0.05) in midguts of females transformed with plasmids encoding catalytically active MEK (pMEK2) or catalytically active MEK K3M/K6M (pMEK5), respectively. Variant MEK allele expression levels in the carcass of transformed mosquitoes were comparable to endogenous MEK allele expression levels in NT female mosquitoes. Five independent mosquito cohorts were used in this experiment.

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