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Table 1 pMEK plasmid nucleotide changes to D-site lysines and catalytic site serines

From: Engineered single nucleotide polymorphisms in the mosquito MEK docking site alter Plasmodium berghei development in Anopheles gambiae

Domain

Codon Position

wtMEK

pMEK1

pMEK2

pMEK3

pMEK4

pMEK5

Docking (D)-Site

3

AAA (K)

AAA (K)

AAA (K)

ATG (M)*

AAA (K)

ATG (M)*

6

AAA (K)

AAA (K)

AAA (K)

AAA (K)

ATG (M)*

ATG (M)*

Catalytic

243

TCA (S)

GAA (E)*

GAA (E)*

GAA (E)*

GAA (E)*

GAA (E)*

 

247

TCT (S)

TCT (S)

GAT (D)*

GAT (D)*

GAT (D)*

GAT (D)*

  1. Mutations at positions 3 and 6 in the D-site were introduced to disrupt MEK-ERK interaction and corresponding phosphorylation of ERK. Mutations at positions 243 and 247 mimic MEK phosphorylation and, hence, activation. The codons are shown with encoded amino acids in parentheses. wtMEK = wild type MEK; amino acid substitutions are noted with asterisks.
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Parasites & Vectors

ISSN: 1756-3305

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