Figure 2

Sequence and predicted structure of mature Sj-L6L-1. A: The Sj-L6L-1 sequence aligned with the highly similar orthologous sequences from S. mansoni (Sm-CD59.2) and S. haematobium (Sh-L6L-1) and compared with Ly-6 family proteins from mice (Mm-Ly-6D) and humans (Hs-E48 and Hs-CD59). The N-terminal signal peptide and the C-terminal propeptide are highlighted in grey, while structurally important cysteine residues are highlighted in red. The predicted omega site (GPI-anchor site) is highlighted in blue. The predicted mature Sj-L6L-1 sequence is indicated within the black triangles. B: The predicted structure of mature Sj-L6L-1 was modelled based on a Phyre2 structure homology search, revealing a structure with the typical three-finger fold common to Ly-6 family proteins. Each finger is labelled with green roman numerals. C: The mature Sj-L6L-1 amino acid sequence has the predicted disulfide bonding pattern, also characteristic of this family, including the GPI-anchor at the C-terminus. The disulfide bonds are shown and numbered in magenta, with those predicted by the in silico analysis depicted by solid lines and those manually inferred by dotted lines.