Table 1 Oligonucleotide primers used for the amplification and sequencing of Babesia orientalis HSP90-A and HSP90-B genes

BoHSP90-A gene

1HSP90-F^a

ATGACGGCTGGGGGAGCTCTG

Primers for amplification of ORF

1HSP90-R ^b

TTAAGCAGCCACAGTGGGTGATTGC

1HSP90F1^a

CAAAACGAGCAGACCTTCCC

Primers for Sequencing

1HSP90F2^a

CCCGAATGTGACGACTATTTGGA

1HSP90F3^a

CTTTTAAGGCTTATGATGACCC

1HSP90F4^a

GCGCTTGAACCTGTTGATGAG

1HSP90R1 ^b

GCCGTATCAAGACTTTCTGCCT

1HSP90R2 ^b

GAAACCAACACCGAACTGACC

1HSP90R3 ^b

TAACATGTTCCCATTCGTGACG

1HSP90R4 ^b

CGGGTTGTTTTGGCTTCATACG

BoHSP90-B gene

2HSP90-F^a

ATGAAGCGGTCCATATTTTTC

Primers for amplification of ORF

2HSP90-R ^b

TCAAAGTTCGTCATTGACGG

2HSP90F1^a

GGCTATTAGGATCAGGGTATCT

Primers for Sequencing

2HSP90F2^a

TATCAGCGGTTCCCATTCAAAC

2HSP90F3^a

CAAGAAGAAGCTCCAGGAAGA

2HSP90F4^a

GAAGGATGACGATATGAACTC

2HSP90R1^b

TCCCGATTTGGCAATTGTT

2HSP90R2 ^b

TGTTTTCGATGCTCTCTTTCAT

2HSP90R3 ^b

CCCCAGTCAGAAACAACAACA