Intravascular persistence of Anaplasma platys, Ehrlichia chaffeensis, and Ehrlichia ewingii DNA in the blood of a dog and two family members

Table 2 Summary of conventional PCR amplification and DNA sequencing results from whole blood, serum, WBC fractions and DH82, RF/6A or IES6 cell cultures tested between September 2011 and June 2012

Date	Mother			Dog				Daughter				Father/Grandmother
	*Ech*	*Eew*	*Apl*	*Ech*	*Eew*	*Apl*	Ec	*Ech*	*Eew*	*Apl*	Ec	*Ech*	*Eew*	*Apl*	Ec
09/2011	+#	-	-	NT	NT	NT	NT	NT	NT	NT	NT	NT	NT	NT	NT
12/2011	+#	+*	+*	+#	-	-	-	NT	NT	NT	NT	NT	NT	NT	NT
01/2012	+#	+#	+#*	+#	-	+#*	-	-	+#	-	-	NT	NT	NT	NT
02/2012	+#	+#	-	-	+#	+*	-	-	-	+*	-	-	-	-	-
03/2012	+*	-	-	-	-	-	+*	+*	-	+*	-	-	-	-	-
06/2012**	-	-	-	-	-	-	-	-	-	-	-	NT	NT	NT	NT

Sample source or sources are designated for each testing time point.
Ech = Ehrlichia chaffeensis, Eew = Ehrlichia ewingii, Apl = Anaplasma platys, Ec = Ehrlichia canis.
All + results confirmed by DNA sequencing.
# = amplicon obtained from blood, serum or buffy coat fraction.
* = amplicon obtained from DH82, RF/6A or IES6 cell cultures.
NT = not tested.
** = post doxycycline treatment.

ISSN: 1756-3305