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Table 2 Summary of conventional PCR amplification and DNA sequencing results from whole blood, serum, WBC fractions and DH82, RF/6A or IES6 cell cultures tested between September 2011 and June 2012

From: Intravascular persistence of Anaplasma platys, Ehrlichia chaffeensis, and Ehrlichia ewingii DNA in the blood of a dog and two family members

Date Mother Dog Daughter Father/Grandmother
  Ech Eew Apl Ech Eew Apl Ec Ech Eew Apl Ec Ech Eew Apl Ec
09/2011 +# - - NT NT NT NT NT NT NT NT NT NT NT NT
12/2011 +# +* +* +# - - - NT NT NT NT NT NT NT NT
01/2012 +# +# +#* +# - +#* - - +# - - NT NT NT NT
02/2012 +# +# - - +# +* - - - +* - - - - -
03/2012 +* - - - - - +* +* - +* - - - - -
06/2012** - - - - - - - - - - - NT NT NT NT
  1. Sample source or sources are designated for each testing time point.
  2. Ech = Ehrlichia chaffeensis, Eew = Ehrlichia ewingii, Apl = Anaplasma platys, Ec = Ehrlichia canis.
  3. All + results confirmed by DNA sequencing.
  4. # = amplicon obtained from blood, serum or buffy coat fraction.
  5. * = amplicon obtained from DH82, RF/6A or IES6 cell cultures.
  6. NT = not tested.
  7. ** = post doxycycline treatment.