Figure 2
Effect of the vaccination in murine leishmaniasis due to L. amazonensis or L. chagasi infection . Mice (four per group) immunized with vaccine diluent (Saline) with CpG-ODN adjuvant (CpG) or with the recombinant vaccine (LmL3 + LmL5 + CpG) were independently challenged subcutaneously in the left footpad with 1 × 106 stationary-phase promastigotes of L. amazonensis (A-B) or 1 × 107 stationary-phase promastigotes of L. chagasi (C). (A) Lesion development was monitored weekly during nine weeks in mice infected with L. amazonensis. The mean ± standard deviation (SD) of the footpad swelling is given as the difference of thickness between the infected and the uninfected contralateral footpad. P values (saline immunized versus vaccinated or CpG-ODN immunized versus vaccinated) are included in the table below the graph. (B) Parasite burden was determined in the infected footpads (parasites per mg) nine weeks after infection with L. amazonensis. Mean + SD is shown. * P < 0.05 indicates significant decrease in the footpad parasite burdens between vaccinated and both control groups. (C) Parasite burdens (parasites per organ) in the spleen, in the liver and in the popliteal lymph node draining the infected footpad (DLN) after L. chagasi infection. Mean + SD of four mice in each group is shown. P values (saline immunized versus vaccinated or CpG-ODN immunized versus vaccinated) are included in the table at the right of the graph. Data shown are representative of two independent experiments with similar results.