Humoral response against the recombinant antigens following infection with L. amazonensis (A) or L. chagasi (B). Nine weeks after parasite challenge vaccinated and control mice (n = 4 per group) were bled and sera were individually tested by ELISA for specific anti-LmL3 and anti-LmL5 antibody responses of both IgG1 and IgG2a isotype at 1/200 dilution. Each bar represents the mean + standard deviation (SD) of data from four individual mice. * P < 0.05 indicates significant increase in the optical density between LmL3 + LmL5 + CpG-ODN vaccinated mice and both control groups. Data shown are representative of two independent experiments with similar results.