Humoral response against soluble parasite antigens (SLA) antigens following infection with L. amazonensis or L. chagasi . Nine weeks after parasite challenge vaccinated and control mice (n = 4 per group) were bled and sera were individually tested by ELISA for specific anti-SLA antibody responses of both IgG1 and IgG2a isotypes at 1/200 dilution. Each bar represents the mean + standard deviation (SD) of data from four individual mice. * P < 0.05 indicates significant changes in the optical density between LmL3 + LmL5 + CpG-ODN vaccinated mice and both control groups. Data shown are representative of two independent experiments with similar results.