Effects of insemination on the locomotor activity of Aedes aegypti (A) and Aedes albopictus (B). Locomotor activity of virgin/unfed (black line; n = 32 for Ae. aegypti and n = 24 for Ae. albopictus) and of inseminated/unfed (red line) Ae. aegypti females (A; n = 32) and (blue line) Ae. albopictus females (B; n = 24) exposed to two days of LD 12:12, at 25°C. First and second days represent, respectively, 48 and 72 hours after insemination. White column represents the photophase and grey column the scothophase. Some reading time points exceeding the scale are indicated with letters, and their values are as follows: a (virgin/unfed) = 23.97 (+7.69); b (inseminated/unfed) = 25.15 (+6.61); c = 25.90 (+8.68).