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Figure 2 | Parasites & Vectors

Figure 2

From: Phylogenetic study of six species of Anopheles mosquitoes in Peninsular Malaysia based on inter-transcribed spacer region 2 (ITS2) of ribosomal DNA

Figure 2

Gel pictures showing PCR amplicons generated from the six Anopheles species using ITS2A and ITS2B primers. Different species show amplicons of different sizes. Lane L1 denotes lane with DNA ladders of 100 bp whereas lane L2 indicates lane with DNA ladders of 1000 bp. The right-most lane of each picture represents the “no-template control”. Agarose gels of 1.5 % (A-C) and 1.3 % (D) were used. The amplicons of An. maculatus (lane 1 – 4) and An. karwari (lane 5 – 12) were shown to be 460 bp and 526 bp respectively (A). For An. peditaeniatus (lane 1) and An. sinensis (lane 2 & 3), amplicons were of 583 bp and 575 bp respectively (B). Specimens of An. cracens (lane 1 – 5) were shown to yield amplicons of 855 bp (C). Amplicons of An. barbirostris specimens (lane 1 & 2) were 1576 bp long (D).

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Parasites & Vectors

ISSN: 1756-3305

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