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Figure 1 | Parasites & Vectors

Figure 1

From: Monoclonal antibody targeting complement C9 binding domain of Trichinella spiralis paramyosin impairs the viability of Trichinella infective larvae in the presence of complement

Figure 1

Recognition of recombinant and native Ts -Pmy (~102 kDa) by mAb 9G3, as demonstrated by Western blot. M, protein marker; Lane 1, adult extracts (5 μg); Lane 2, ML extracts (5 μg); Lane 3, NBL extracts (5 μg); Lane 4, rTs-Pmy with a His-tag (0.5 μg); Lane 5,BSA(1 μg); Lane 6, irrelevant recombinant Ts-Hsp70 control (1 μg). After transfer of the proteins to the nitrocellulose membrane, the membrane was incubated with mAb 9G3 (0.2 μg/ml). IRDye 800CW-labeled goat anti-mouse IgG was used as the secondary antibody.

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