Table 1 Statistics of RNA-seq based sequencing, assembling and functional annotation for An. sinensis
Sequencing results | Number of total raw reads | 60,866,926 |
|---|---|---|
| Â | Number of total clean reads | 51,606,364 |
| Â | Number of total clean nucleotides (nt) | 4,644,572,760 |
| Â | Q20 percentage of total clean reads | 95.92% |
| Â | GC percentage of total clean nucleotides | 51.26% |
| Â | N percentage of total clean nucleotides | 0.00% |
Assembling results | Number of unigenes | 38,504 (5,372 into distinct clusters; 33,132 singletons) |
| Â | Total length (nt) of total unigenes | 21,977,286 |
| Â | Mean length (nt) of total unigenes | 571 |
| Â | N50 (nt) of total unigenes | 711 |
Annotation | Unigenes with Nr database | 25,456 (66% of 38,504 unigenes) |
(E-value < =1e-5) | Unigenes with Nt database | 20,554 (53%) |
| Â | Unigenes with Swiss-Prot database | 17,651 (46%) |
| Â | Unigenes with KEGG database | 16,622 (43%), 257 pathways |
| Â | Unigenes with COG database | 7,204 (19%), 25 functional categories |
| Â | Unigenes with GO database | 16,588 (43%), 62 subcategories grouped to 3 main categories |
| Â | Biological process | 27 sub-categories |
| Â | Cellular component | 17 sub-categories |
| Â | Molecular function | 18 sub-categories |
| Â | Total unigenes annotated | 26,650 (69% of 38,504 unigenes) |