Amplification curves in the multiplex real-time PCR for two mosquitoes positive for P. vivax . (A) An. 1, Anopheles dirus; (B) An. 2, Anopheles pampanai; and (C) amplification of cytochrome oxidase cox I fragment by conventional PCR of P. vivax detected in Anopheles females collected in Vietnam. Pf and Pv = positive controls for P. falciparum and P. vivax.