Table 1 Sequences of primers and probes used for the detection of Plasmodium species and W. bancrofti using multiplex real-time PCR
Name | Sequence (5′ - 3′) | Target |
|---|---|---|
Plasmo 1 | GTTAAGGGAGTGAAGACGA TCAGA | Plasmodium sp. and P. knowlesi |
Plasmo 2 | AACCCAAAGACTTTGATTTC TCATAA | |
Plasprobe | FAM-ACCGTCGTAATCTTAACCATAAACTATGCCGACTAG-BHQ1 | |
Pk probe | HEX-CTCTCCGGAGATTAGAACTCTTAGATTGCT-BHQ1 | |
FAL_F | CTT TTG AGA GGT TTT GTT ACT TTG AGT AA | Plasmodium falciparum |
FAL_R | TAT TCC ATG CTG TAG TAT TCA AAC ACA A | |
FAL_PB | FAM - TGT TCA TAA CAG ACG GGT AGT CAT GAT TGA GTT CA - BHQ1 | |
VIV_F | ACG CTT CTA GCT TAA TCC ACA TAA CT | Plasmodium vivax |
VIV_R | ATT TAC TCA AAG TAA CAA GGA CTT CCA AGC | |
VIV_PB | HEX - TTC GTA TCG ACT TTG TGC GCA TTT TGC - BHQ1 | |
LDR1_F | ATT TTG ATC ATC TGG GAA CGT TAA TA | Wuchereria bancrofti |
LDR2_R | CGA CTG TCT AAT CCA TTC AGA GTG A | |
WB_PB | Cy5 - ATC TGC CCA TAG AAA TAA CTA CGG TGG ATC TCT G - BHQ2 |