Table 1 Details of primers, probe, product sizes and gene positions in PCR assays and sequencing of rickettsial genes
Gene | Primers and probe | Nucleotide sequence (5’ to 3’) | Product size (bp) |
|---|---|---|---|
gltA | CS-F | TCG CAA ATG TTC ACG GTA CT | 74 |
CS-R | TCG TGC ATT TCT TTC CAT TGT G | ||
CS-P | 6-FAM-TGC AAT AGC AAG AAC CGT | Probe | |
AGG CTG GAT G--BBQ-1 | |||
ompB | Rc.rompB.4362p | GTC AGC GTT ACT TCT TCG ATG C | 475 |
Rc.rompB.4,836n | CCG TAC TCC ATC TTA GCA TCA G | ||
Rc.rompB.4,496p | CCA ATG GCA GGA CTT AGC TAC T | 267 | |
Rc.rompB.4,762n | AGG CTG GCT GAT ACA CGG AGT AA | ||
17 kDa | Rr17kDa.61p | GCT CTT GCA ACT TCT ATG TT | 434 |
Rr17kDa.492n | CAT TGT TCG TCA GGT TGG CG | ||
ompA | Rr 190.70 F | ATG GCG AAT ATT TCT CCA AAA | 632 |
Rr 190.701R | GTT CCG TTA ATG GCA GCA TCT | ||
gltA | RH314 | AAA CAG GTT GCT CAT CAT TC | 857 |
CSF-R | AAG TAC CGT GAA CAT TTG CGA | ||
CS-Ric-R | CAG TGA ACA TTT GCG ACG GTA | 852 | |
CS535d | GCA ATG TCT TAT AAA TAT TC | Sequencing | |
Primer |