Skip to main content

Advertisement

Table 1 Details of primers, probe, product sizes and gene positions in PCR assays and sequencing of rickettsial genes

From: Spotted fever Rickettsia species in Hyalomma and Ixodes ticks infesting migratory birds in the European Mediterranean area

Gene Primers and probe Nucleotide sequence (5’ to 3’) Product size (bp)
gltA CS-F TCG CAA ATG TTC ACG GTA CT 74
  CS-R TCG TGC ATT TCT TTC CAT TGT G  
  CS-P 6-FAM-TGC AAT AGC AAG AAC CGT Probe
   AGG CTG GAT G--BBQ-1  
ompB Rc.rompB.4362p GTC AGC GTT ACT TCT TCG ATG C 475
  Rc.rompB.4,836n CCG TAC TCC ATC TTA GCA TCA G  
  Rc.rompB.4,496p CCA ATG GCA GGA CTT AGC TAC T 267
  Rc.rompB.4,762n AGG CTG GCT GAT ACA CGG AGT AA  
17 kDa Rr17kDa.61p GCT CTT GCA ACT TCT ATG TT 434
  Rr17kDa.492n CAT TGT TCG TCA GGT TGG CG  
ompA Rr 190.70 F ATG GCG AAT ATT TCT CCA AAA 632
  Rr 190.701R GTT CCG TTA ATG GCA GCA TCT  
gltA RH314 AAA CAG GTT GCT CAT CAT TC 857
  CSF-R AAG TAC CGT GAA CAT TTG CGA  
  CS-Ric-R CAG TGA ACA TTT GCG ACG GTA 852
  CS535d GCA ATG TCT TAT AAA TAT TC Sequencing
    Primer