Humoral immune profile of experimentally challenged rodents against rSjB6. Mice (n = 5) and rats (n = 3) were experimentally challenged with S. japonicum cercariae and immune sera collected six weeks post infection with additional sera collected from the rats 6 weeks post-secondary challenge. Pre-immune sera were also collected prior to the cercarial challenge. Each data point represents an average of 3 replicates. There was no significant difference between the serum antibody levels in the mice before and after challenge (left panel). Rat sera showed high serum reactivity against rSjB6 after challenge (right panel). Error bars represent mean + SEM. 1° = primary challenge, 2° = secondary challenge, PRE = pre-immune sera. Statistically significant difference (Two-way ANOVA: ****= p-value < 0.0001, ***= p-value 0.0015).