Sequence analysis of Aalb_CTL1. (A) ORF and deduced amino acid sequence of Aalb_CTL1 from the Aedes albopictus Guangzhou strain. The amino acid sequence is represented by a single capital letter below the nucleotide sequence. The putative signal peptide sequence is underlined; four conserved cysteine residues that define the C-type lectin domain are shaded and underlined. The EPS motif for ligand binding specificity is in box. The WND motif is in double lines. The mutation position is in red. (B) Comparison of a putative CRD of Aalb_CTL1 with known and putative mosquito C-type lectins. Alignment of the CRD domain from Aalb_CTL1, Aaeg CTL16 [VectorBase: AAEL000533-RA], Aaeg CTL20 [VectorBase: AAEL011407-RA], Aaeg CTL25 [VectorBase: AAEL000556-RA], Aaeg mosGCTL-1 [VectorBase: AAEL000563-RA], Aalb_CTL2 [Genbank: AAV90641], Cq CTL54 [VectorBase: CPIJ014105], Agam CTL4 [VectorBase: ENSANGG00000018677], Agam CTLMA2 [VectorBase: ENSANGG00000018421]. Invariant or highly conserved residues within the CRD [Drickamer, 1993] are shown in the upper row: the italic single-letter amino acid codes indicate invariant conserved amino acids. Other abbreviations: Θ, aliphatic; Φ, aromatic, Ω aliphatic or aromatic; O, oxygen-containing; Z, Glutamine or glutamic acid; triangle: the position for mutation. Cysteine residues forming disulphide bonds are marked with asterisks. Numbers on the left indicate amino acid positions starting from the initial methionine.