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Figure 4 | Parasites & Vectors

Figure 4

From: Cloning and characterization of a mannose binding C-type lectin gene from salivary gland of Aedes albopictus

Figure 4

Expression and purification of recombinant mature Aalb_CTL1 (rAalb_CTL1). Lane M: protein molecular standard. Lane 1: pET-28a(+) in BL21, uninduced. Lane 2: pET-28a(+) in BL21, IPTG-induced for 5 h. Lane 3: pET-28a(+)-Aalb_CTL1 in BL21, uninduced. Lane 4: pET-28a(+)-Aalb_CTL1 in BL21, IPTG-induced for 5 h. Lane 5: Supernatants of lysate of BL21 cell containing pET-28a(+)-Aalb_CTL1 induced with IPTG. Lane 6: Sediments of lysate of BL21 cell containing pET-28a(+)-Aalb_CTL1 induced with IPTG. Lane 7: pET-28a(+)-Aalb_CTL1 recombinant proteins purified by the affinity purification method.

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