Figure 6

Inhibitory effect of rHco-gal-m on T cell activation and proliferation. (A) T cells were activated with ConA and incubated at the same time with serial concentrations of rHco-gal-m at 37°C and 5% CO₂. The proliferation was measured by CCK-8 incorporation after 72 h. Cell proliferation index was calculated considering the OD₄₅₀ values in controls as 100%. (B) T cells were simultaneously treated with ConA and rHco-gal-m at 37°C and 5% CO₂ for 24 h. The expression of CD25, CCNA1, CCNB1 and CCND1 was analyzed by real-time PCR. All experiments were set up in triplicate. The data are representative of three independent experiments (*p < 0.05 and **p < 0.01).