Table 1 Primer sequences of target genes in real-time PCR assay
Gene | GenBank | Forward primer | Reverse primer | Amplicon |
|---|---|---|---|---|
Name | Accession | 5′ → 3′ | 5′ → 3′ | Size (bp) |
CD25 | NM_174358 | AGATGTCCTGGCTTGAAC | TGGATATAGACCTGCTAATACC | 134 |
CCNA1 | XM_005687507 | CGCCACATTTACAGGCTATTCATT | GTACTTCTCCCTGATTGCTTGCTG | 112 |
CCNB1 | XM_005694606 | TTGATGGAACTGACTATGCTGGACT | CAGGTAATGCTGTAGAGTTGGTGTC | 132 |
CCND1 | XM_005700049 | AGGAGGTGAGGGTGGAAGTGTG | GAAGTCATCGGTAGCAGCGAATAA | 100 |
GAPDH | DQ152956 | CCTGGAGAAACCTGCCAAGT | GCCAAATTCATTGTCGTACCA | 200 |