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Table 1 The physiological and biochemical responses of nymphal H . longicornis after low-temperature treatments

From: Cold hardiness and biochemical response to low temperature of the unfed bush tick Haemaphysalis longicornis (Acari: Ixodidae)

Treatment

Physiological and biochemical response (nymph)

Survival rate (%)

Super-cooling point (°C)

Water (%)

Glycerol (μg/mL)

Protein (μg/mL)

Control

100 ± 0.0

-22.7 ± 1.4

57.6 ± 1.0

2.6 ± 0.1

68.2 ± 7.9

0°C 2 h

100 ± 0.0

-23.8 ± 0.9*

59.3 ± 1.6

2.9 ± 1.1

53.9 ± 12.7

5°C 2 h

100 ± 0.0

-24.0 ± 1.4*

64.8 ± 1.1**

2.9 ± 0.3

61.0 ± 8.6

0°C 10d

62.4 ± 5.6

-23.3 ± 1.4

54.7 ± 2.2

4.2 ± 0.1**

112.5 ± 4.6**

0°C 20d

54.3 ± 7.8

-23.7 ± 1.1*

50.3 ± 1.6**

3.5 ± 0.3**

21.8 ± 6.6**

  1. *p < 0.05, **p < 0.01.