Figure 3

Alignment of the cytochrome c oxidase subunit I (COI) gene derived from seven samples. Position of the forward primer (Anop_COI_F) and biotinylated reverse primer (Anop_COI_Rb) for template amplification are shown in the black boxes, while the sequencing primer (Anop_COI_S) and target region are shown in the green and red boxes, respectively. Asterisks indicate position of the target region used for species level identification.