Circulation of four Anaplasma phagocytophilum ecotypes in Europe

Parasites & Vectors

Table 3 Presence of A. phagocytophilum in vertebrate tissue samples

Species	Common name	Tested (n)	Positive (n)
Apodemus flavicollis	Yellow-necked mouse	2	0
*Apodemus sylvaticus*	Wood mouse	23	1
Crocidura russula	White-toothed shrew	5	0
Microtus arvalis	Common vole	8	0
Myodes glareolus	Bank vole	35	0
Sorex araneus	Common shrew	6	0
Carduelis chloris	Greenfinch	4	0
Coccothraustes coccothraustes	Hawfinch	2	0
Fringilla coelebs	Common chaffinch	3	0
Parus major	Great tit	4	0
Phylloscopus trochilus	Willow warbler	1	0
Pyrrhula pyrrhula	Bullfinch	1	0
Turdus iliacus	Redwing	5	0
*Turdus merula*	Common blackbird	11	1
Turdus philomelos	Song thrush	6	0
*Capreolus capreolus*	Roe deer	38	26
Equus ferus caballus	Domestic horse	14	14
*Vulpes vulpes*	Red fox	81	8
Meles meles	Badger	40	0
Total		289	50

DNA extracts from spleen and EDTA-blood of wildlife and horses were tested by qPCR. The presence of A. phagocytophilum was confirmed in most cases by conventional PCR using groEL specific primer pairs, followed by sequencing. Positive animal species are shown in bold.

ISSN: 1756-3305