Figure 8
From: Activated microglia contribute to neuronal apoptosis in Toxoplasmic encephalitis
Expression of iNOS protein in BV-2 cells. The three groups include mouse microglia BV-2 cells (control), T.gondii-infected BV-2 cells (T.gondii) or BV-2 cells infected with T.gondii and treated with minocycline (T.gondii + M). (A) BV-2 cells were fixed and stained with antibodies against iNOS (red) and tachyzoite surface antigen SAG-1(green) 24 h after infection with T.gondii. Hoechst dye was used to stain nuclei (blue). Double immunofluorescence showed iNOS expression in parasite-infected BV-2 cells. (B) Western blotting analysis of iNOS. Histograms show densitometric analyses of the relative level of iNOS protein normalized over β-actin expression. Values represent the means ± SEM of six animals per group. (*P < 0.01 vs Control; #P < 0.01 vs TE).