Figure 2

Generation of an Aedes vexans cell line of UK origin using embryos as the source material. The first passage (P) was undertaken 14 days (d) after adding crushed embryos to cell media and P2 was undertaken 7d after P1 to allow cells to adhere. Routine passage of the cell line was undertaken from P3 by transferring 20% cells in media to 80% media. A) P1 after 14d showing formation of a high density monolayer. B) P2 after 7d showing the start of a monolayer forming. C) P3 after 1d and D) P3 after 4d showing growth of the cell line.