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Table 2 Identified peptides of C. punctatus using offline nano-HPLC-MALDI-TOF-MS/MS and nano-HPLC-Orbitrap-MS/MS

From: Species determination of Culicoides biting midges via peptide profiling using matrix-assisted laser desorption ionization mass spectrometry

  

LC-MALDI

LC-Orbitrap

  

Name

Sequence

MH+ [Da]

Δ m[ppm]

S/N

RT[min]

MH+[Da]

Δ m[ppm]

RT[min]

Modification

Identical to peptide in species of the genus:

Actin

AVFPSIVGRPR

1198.71

3.398

6835.5

39.65

1198.707

0.971

37.26

 

Droso., Culex, Bact., Aedes, Anoph., Belgica, Cerat.

Actin

GYSFTTTAEREIVR

1629.82

−3.862

472.8

40.02

1629.826

1.764

38.76

 

Maye., Culex, Culi., Bact., Psoro., Chiro., Anoph., Ochler., Wyeo., Simu., Teleo.

Actin

SYELPDGQVITIGNER

1790.89

2.934

1177.2

50.08

1790.897

2.800

47.17

 

Anas., Chiro., Teleo., Bact., Culi., Anoph., Calli., Simu., Psoro., Manso., Pseudo., Droso., Aedes, Belgica, Culex, Cerat.

Actin

VAPEEHPVLLTEAPLNPK

1954.06

28.501

1477.5

43.24

1954.068

1.648

42.42

 

Anoph., Lirio., Droso., Culex, Bact., Aedes, Belgica, Cerat.

Actin

DLYANSVLSGGTTMYPGIADR

2201.05

17.826

285.1

53.18

2201.064

4.359

53.34

 

Anas., Bact., Anoph., Droso., Aedes, Culex

Actin

TTGIVLDSGDGVSHTVPIYEGYALPHAILR

3151.64

23.668

645.9

56.12

3151.655

4.088

54.96

 

Simu., Clui., Anoph., Psoro., Culex, Ochler., Manso., Toxor., Chiro., Lirio., Teleo., Bact., Aedes, Droso.

ATP-Synthase alpha unit (mit.)

VSVREPMQTGIK

1344.73

−2.340

217.4

28.05

1344.731

0.669

26.87

 

Cerat., Anoph., Aedes., Culex, Droso.

ATP-Synthase alpha unit (mit.)

TALAIDTIINQQR

1456.81

−19.470

171.9

48.78

1456.819

5.182

48.99

 

Aedes aegypti

ATP-Synthase alpha unit (mit.)

EAYPGDVFYLHSR

1553.74

29.590

374.7

43.20

1553.742

2.022

42.11

 

Droso., Anoph., Culex., Aedes, Cerat.,

ATP-Synthase alpha unit (mit.)

TGAIVDVPVGDELLGR

1610.88

11.787

158.8

53.04

1610.880

3.067

53.34

 

Droso., Aedes, Cerat.

ATP-Synthase beta unit (mit.)

FTQAGSEVSALLGR

1435.75

−61.429

314.7

48.38

1435.758

2.830

48.06

 

Droso., Sarco., Cerat., Aedes

ATP-Synthase beta unit (mit.)

VALVYGQMNEPPGAR

1601.81

8.391

454.4

38.77

1601.816

3.312

37.69

 

Droso., Sarco., Cerat. Aedes

ATP-Synthase beta unit (mit.)

VALVYGQMNEPPGAR

1617.81

−13.445

137.7

32.82

1617.808

1.453

31.54

M8 (Oxidation)

Droso., Aedes, Cerat.

ATP-Synthase beta unit (mit.)

AIAELGIYPAVDPLDSTSR

1988.03

29.102

87.8

55.50

1988.042

4.384

55.69

 

Droso., Cerat., Culex, Aedes, Anoph.

ATP-Synthase beta unit (mit.)

VLDTGYPIRIPVGAETLGR

2027.13

−3.416

395.8

52.22

2027.133

2.086

52.20

 

Droso., Cerat.

ATP-Synthase beta unit (mit.)

IPSAVGYQPTLATDMGTMQER

2266.08

−54.675

220.4

48.05

2266.094

4.281

48.07

 

Drosophila virilis

Myosin

LSIENSDLLR

1159.63

31.037

191.0

43.16

1159.632

0.509

42.42

 

Droso., Anoph., Aedes, Culex

Myosin

AFDKIIGEWK

1206.65

−41.827

234.5

48.73

1206.652

−0.017

44.71

 

Anoph., Droso., Culex, Bact., Aedes, Cerat.

Myosin

VRELENELDGEQR

1586.78

16.930

273.4

29.40

1586.777

−0.266

27.03

 

Droso., Cerat.

Myosin

LKGAYEEGQEQLEAVR

1819.92

−49.529

476.0

34.48

1819.919

0.008

32.13

 

Droso., Culex, Cerat., Anoph., Aedes, Bact.

Myosin

NLADEVKDLLDQIGEGGR

1941.99

−10.703

46.7

64.00

1941.996

4.029

63.64

 

Droso., Culex, Anoph., Aedes, Cerat., Bact.

Myosin

LKVDDLAAELDASQKECR

2061.03

−0.848

353.1

45.20

2061.038

0.836

42.54

C17 (Carbamidomethyl)

Droso., Culex, Cerat., Anoph., Aedes, Bact.

Myosin

AKLEQTLDELEDSLEREK

2146.09

−0.126

166.2

57.37

2146.094

2.959

54.49

 

Dros., Culex, Cerat., Aedes, Anoph.

Myosin

TALLDSLSGEKGALQEYQEK

2180.11

−47.922

94.5

46.60

2180.114

2.789

46.41

 

Anoph., Culex, Aedes

Myosin

GSLEDQVVQTNPVLEAFGNAK

2216.12

18.550

83.9

56.62

2216.123

1.770

56.65

 

Droso., Anoph., Cerat.

Myosin

AQQELEEAEERADLAEQAISK

2358.14

−49.243

841.3

47.22

2358.148

2.661

47.08

 

Droso., Cerat.

Myosin

AQQELEEAEERADLAEQAISKFR

2661.31

25.032

37.3

53.59

2661.323

4.107

53.49

 

Droso., Cerat.

Tropomyosin

SLADEERMDALENQLKEAR

2218.08

26.636

46.3

44.13

2218.081

1.916

43.17

 

Chiro., Culex

  1. Table contains only those identified peptides visible as a peak in the SMM spectrum obtained using unfractionated extracts. Droso. : Drosophila, Bact.: Bactocera, Anoph.: Anopheles, Cerat.: Ceratitis, Maye.: Mayetiola, Culi.: Culicoides, Psoro.: Psorophora, Chiro.: Chironomus, Ochler.: Ochlerotatus, Wyeo.: Wyeomyia, Simu.: Simulium, Teleo.: Teleopsis, Anas.: Anastrepha, Calli.: Calliphora, Manso.: Mansonia, Toxor.: Toxorhynchites, Pseudo.: Pseudodiamesa, Lirio.: Liriomyza, Sarco.: Sarcophaga.
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Parasites & Vectors

ISSN: 1756-3305

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