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Table 2 Identified peptides of C. punctatus using offline nano-HPLC-MALDI-TOF-MS/MS and nano-HPLC-Orbitrap-MS/MS

From: Species determination of Culicoides biting midges via peptide profiling using matrix-assisted laser desorption ionization mass spectrometry

   LC-MALDI LC-Orbitrap   
Name Sequence MH+ [Da] Δ m[ppm] S/N RT[min] MH+[Da] Δ m[ppm] RT[min] Modification Identical to peptide in species of the genus:
Actin AVFPSIVGRPR 1198.71 3.398 6835.5 39.65 1198.707 0.971 37.26   Droso., Culex, Bact., Aedes, Anoph., Belgica, Cerat.
Actin GYSFTTTAEREIVR 1629.82 −3.862 472.8 40.02 1629.826 1.764 38.76   Maye., Culex, Culi., Bact., Psoro., Chiro., Anoph., Ochler., Wyeo., Simu., Teleo.
Actin SYELPDGQVITIGNER 1790.89 2.934 1177.2 50.08 1790.897 2.800 47.17   Anas., Chiro., Teleo., Bact., Culi., Anoph., Calli., Simu., Psoro., Manso., Pseudo., Droso., Aedes, Belgica, Culex, Cerat.
Actin VAPEEHPVLLTEAPLNPK 1954.06 28.501 1477.5 43.24 1954.068 1.648 42.42   Anoph., Lirio., Droso., Culex, Bact., Aedes, Belgica, Cerat.
Actin DLYANSVLSGGTTMYPGIADR 2201.05 17.826 285.1 53.18 2201.064 4.359 53.34   Anas., Bact., Anoph., Droso., Aedes, Culex
Actin TTGIVLDSGDGVSHTVPIYEGYALPHAILR 3151.64 23.668 645.9 56.12 3151.655 4.088 54.96   Simu., Clui., Anoph., Psoro., Culex, Ochler., Manso., Toxor., Chiro., Lirio., Teleo., Bact., Aedes, Droso.
ATP-Synthase alpha unit (mit.) VSVREPMQTGIK 1344.73 −2.340 217.4 28.05 1344.731 0.669 26.87   Cerat., Anoph., Aedes., Culex, Droso.
ATP-Synthase alpha unit (mit.) TALAIDTIINQQR 1456.81 −19.470 171.9 48.78 1456.819 5.182 48.99   Aedes aegypti
ATP-Synthase alpha unit (mit.) EAYPGDVFYLHSR 1553.74 29.590 374.7 43.20 1553.742 2.022 42.11   Droso., Anoph., Culex., Aedes, Cerat.,
ATP-Synthase alpha unit (mit.) TGAIVDVPVGDELLGR 1610.88 11.787 158.8 53.04 1610.880 3.067 53.34   Droso., Aedes, Cerat.
ATP-Synthase beta unit (mit.) FTQAGSEVSALLGR 1435.75 −61.429 314.7 48.38 1435.758 2.830 48.06   Droso., Sarco., Cerat., Aedes
ATP-Synthase beta unit (mit.) VALVYGQMNEPPGAR 1601.81 8.391 454.4 38.77 1601.816 3.312 37.69   Droso., Sarco., Cerat. Aedes
ATP-Synthase beta unit (mit.) VALVYGQMNEPPGAR 1617.81 −13.445 137.7 32.82 1617.808 1.453 31.54 M8 (Oxidation) Droso., Aedes, Cerat.
ATP-Synthase beta unit (mit.) AIAELGIYPAVDPLDSTSR 1988.03 29.102 87.8 55.50 1988.042 4.384 55.69   Droso., Cerat., Culex, Aedes, Anoph.
ATP-Synthase beta unit (mit.) VLDTGYPIRIPVGAETLGR 2027.13 −3.416 395.8 52.22 2027.133 2.086 52.20   Droso., Cerat.
ATP-Synthase beta unit (mit.) IPSAVGYQPTLATDMGTMQER 2266.08 −54.675 220.4 48.05 2266.094 4.281 48.07   Drosophila virilis
Myosin LSIENSDLLR 1159.63 31.037 191.0 43.16 1159.632 0.509 42.42   Droso., Anoph., Aedes, Culex
Myosin AFDKIIGEWK 1206.65 −41.827 234.5 48.73 1206.652 −0.017 44.71   Anoph., Droso., Culex, Bact., Aedes, Cerat.
Myosin VRELENELDGEQR 1586.78 16.930 273.4 29.40 1586.777 −0.266 27.03   Droso., Cerat.
Myosin LKGAYEEGQEQLEAVR 1819.92 −49.529 476.0 34.48 1819.919 0.008 32.13   Droso., Culex, Cerat., Anoph., Aedes, Bact.
Myosin NLADEVKDLLDQIGEGGR 1941.99 −10.703 46.7 64.00 1941.996 4.029 63.64   Droso., Culex, Anoph., Aedes, Cerat., Bact.
Myosin LKVDDLAAELDASQKECR 2061.03 −0.848 353.1 45.20 2061.038 0.836 42.54 C17 (Carbamidomethyl) Droso., Culex, Cerat., Anoph., Aedes, Bact.
Myosin AKLEQTLDELEDSLEREK 2146.09 −0.126 166.2 57.37 2146.094 2.959 54.49   Dros., Culex, Cerat., Aedes, Anoph.
Myosin TALLDSLSGEKGALQEYQEK 2180.11 −47.922 94.5 46.60 2180.114 2.789 46.41   Anoph., Culex, Aedes
Myosin GSLEDQVVQTNPVLEAFGNAK 2216.12 18.550 83.9 56.62 2216.123 1.770 56.65   Droso., Anoph., Cerat.
Myosin AQQELEEAEERADLAEQAISK 2358.14 −49.243 841.3 47.22 2358.148 2.661 47.08   Droso., Cerat.
Myosin AQQELEEAEERADLAEQAISKFR 2661.31 25.032 37.3 53.59 2661.323 4.107 53.49   Droso., Cerat.
Tropomyosin SLADEERMDALENQLKEAR 2218.08 26.636 46.3 44.13 2218.081 1.916 43.17   Chiro., Culex
  1. Table contains only those identified peptides visible as a peak in the SMM spectrum obtained using unfractionated extracts. Droso. : Drosophila, Bact.: Bactocera, Anoph.: Anopheles, Cerat.: Ceratitis, Maye.: Mayetiola, Culi.: Culicoides, Psoro.: Psorophora, Chiro.: Chironomus, Ochler.: Ochlerotatus, Wyeo.: Wyeomyia, Simu.: Simulium, Teleo.: Teleopsis, Anas.: Anastrepha, Calli.: Calliphora, Manso.: Mansonia, Toxor.: Toxorhynchites, Pseudo.: Pseudodiamesa, Lirio.: Liriomyza, Sarco.: Sarcophaga.