Figure 2

Phylogenetic tree based on the short NS5 fragment of the Flavivirus genus. A fragment of 214 bp of the NS5 gene was used to infer the tree. The phylogenetic analysis includes 62 reference sequences from GenBank, 3 sequences from the 2011 surveillance program, and 25 from the present study (19 ISF, 4 USUV, 2 MMV). Unpublished sequences are given in bold, Piedmont sequences in italics. The nucleotide substitution model used as a prior for the MCMC was GTR + G, according to the jModelTest2 results. The tree is mid-point routed; for clarity, only posterior probability of the nodes >0.7 is reported in the figure for statistical support. The scale of branch length is expressed as substitution per site. Taxa are divided into four clusters according to the literature: IS insect-specific; NK no-known vector; MB mosquito-borne; TB tick-borne.