Identification of early diagnostic antigens from major excretory-secretory proteins of Trichinella spiralis muscle larvae using immunoproteomics

Wang, Li; Cui, Jing; Hu, Dan Dan; Liu, Ruo Dan; Wang, Zhong Quan

doi:10.1186/1756-3305-7-40

Table 1 Identification of Trichinella spiralis muscle larval major ES proteins recognized by mouse infection sera at 18 dpi using MALDI-TOF/TOF-MS

From: Identification of early diagnostic antigens from major excretory-secretory proteins of Trichinella spiralis muscle larvae using immunoproteomics

Spot no.	Protein name	Species	Accession no.	Theoretical Mr/pI^a	MOWSE score^b	Coverage (%)	No. matched peptides	E-value
1	Serine protease	T. spiralis	gi\|168805931	35.7/5.97	335	22	8	7e-027
2	Alpha S1 casein	Bos taurus	gi\|159793193	18.7/5.23	208	53	8	3.5e-014
3	Putative trypsin	T. spiralis	gi\|339241891	31.3/5.25	132	21	3	1.4e-006
4	Unknown	Legionella rubrilucens	gi\|61814442	12.0/6.84	43	61	5	1e + 003
5	Putative trypsin	T. spiralis	gi\|339241897	53.9/5.97	308	17	8	3.5e-024
6	Serine protease	T. spiralis	gi\|168805931	35.7/5.97	608	28	6	3.5e-054
7	Deoxyribonuclease II family protein	T. spiralis	gi\|339241449	38.1/5.97/	440	30	8	2.2e-037
8	Serine protease	T. spiralis	gi\|13641204	48.7/6.33	337	18	8	4.4e-027
9	Serine protease	T. spiralis	gi\|13641204	48.7/6.33	200	11	3	2.2e-013
10	Serine protease	T. spiralis	gi\|13641204	48.7/6.33	405	17	7	7e-034
11	Serine protease	T. spiralis	gi\|13641204	48.7/6.33	347	15	6	4.4e-028
12	Deoxyribonuclease II family protein	T. spiralis	gi\|339241449	38.1/5.95	195	28	7	7e-013
13	Deoxyribonuclease II family protein	T. spiralis	gi\|339241449	38.1/5.95	336	34	10	5.5e-027
14	Hypothetical protein	Afipia felis	gi\|414163749	35.7/10.2	56	36	9	54
15	Hypothetical protein	Pantoea ananatis	gi\|378767336	35.1/10.9	49	100	4	2.7e + 002
16	Serine protease	T. spiralis	gi\|13641204	48.7/6.33	336	17	8	5.5e-027
17	Serine protease	T. spiralis	gi\|13641204	48.7/6.33	54	14	5	94
18	Serine protease	T. spiralis	gi\|13641204	48.7/6.33	304	11	4	8.8e-024
19	Serine protease	T. spiralis	gi\|13641204	48.7/6.33	443	22	6	1.1e-037
20	Serine protease	T. spiralis	gi\|168805933	48.7/6.33	353	11	9	1.1e-028
21	Serine protease	T. spiralis	gi\|168805933	48.7/6.33	471	20	9	1.8e-040

^aTheoretical molecular mass (kDa) and isoelectric point (pI).
^bA MOWSE score of >70 was used to assign identity to a protein.

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ISSN: 1756-3305

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