Figure 1

Levels of cTXNPx gene transcript in Sb(III)-susceptible and Sb(III)-resistant L. braziliensis and L. infantum lines. (A) Northern blot analysis of total RNA (20 μg) from Leishmania spp. lines separated on a 1.2% agarose gel and transferred to nylon membranes. Blots were hybridized with a ³²P-labelled cTXNPx-specific probe. (B) As a control, the same nylon membrane was hybridized with a ³²P-labelled 24S rRNA-specific probe. (C) Quantification of bands was done by densitometric analysis using the software CP ATLAS 2.0. (D) Levels of cTXNPx mRNA of L. braziliensis and L. infantum lines determined quantitatively (relative to the 18S small subunit ribosomal RNA -18S SSU rRNA) by real-time PCR. Mean values of the transcript levels of cTXNPx/SSU ± standard deviations as determined from three independent experiments are shown. Statistically different values are indicated as follows: *p < 0.04, **p < 0.0002.