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Figure 1 | Parasites & Vectors

Figure 1

From: A pan-Theileria FRET-qPCR survey for Theileria spp. in ruminants from nine provinces of China

Figure 1

Alignment of oligonucleotides for Theileria PCR used in this study. Primers and probes are shown at the top of the boxes. Dots indicate nucleotides identical to primers and probes, and dashes denote absence of the nucleotide. The upstream primer is used as the demonstrated sequences without gaps while the two probes and downstream primer are used as antisense oligonucleotides. The designed oligonucleotides show minimum mismatching with Theileria spp. (0 mismatch with 11 species, 1 mismatch with 3 species, 2 mismatches with 2 species and 4 mismatches with 1 species), but significant numbers of mismatches with Babesia bovis (25 mismatches), B. divergens (23 mismatches), B. bigemina (22 mismatches), Cytauxzoon felis (8 mismatches), Hepatozoon americanum (16 mismatches) and Toxoplasma gondii (15 mismatches). The 6-FAM label is directly attached to the 3-terminal nucleotide of the fluorescein probe, and the LCRed-640 fluorescein label is added via a linker to the 5′-end of the LCRed-640 probe.

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