Table 1 Comparison of medium ingredients between MKP-F and the MKP medium it is based upon

MilliQ

662.8 ml

MilliQ

670 ml

7% gelatine

127.3 ml

7% gelatine

149 ml

FCS

100 ml

-

10× CMRL

65.1 ml

10x CMRL

74.5 ml

Rabbit serum

44.8 ml

Rabbit serum

53.6 ml

BSA

32.8 g

BSA

52.2 ml

HEPES

3.9 g

HEPES

4.5 g

Glucose

3.3 g

Glucose

2.2 g

Neopeptone

2.0 g

Neopeptone

3.7 g

Sodium bicarbonate

1.4 g

Sodium bicarbonate

1.5 g

Sodium citrate

0.5 g

Sodium citrate

0.5 g

Sodium pyruvate

0.5 g

Sodium pyruvate

0.6 g

N-acetyl glucosamine

0.3 g

N-acetyl glucosamine

0.3 g