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Table 2 Primer sequences and main conditions of the PCR methods used

From: Molecular diagnosis of cutaneous leishmaniasis and identification of the causative Leishmania species in Morocco by using three PCR-based assays

    PCR conditions
Primer name Primer sequence Amplification conditions MgCl2(mM) dNTP (mM) Primer (μM) Unit of Taq DNA Polymerase DNA (μL)
13A 5′-GTG GGG GAG GGG CGT TCT-3′ 94°C for 4 min, 94°C for 1 min, 60°C for 1 min, 72°C for 1 min, 72°C for 10 min (30 cycles) 1.5 0.2 1 1 2.5
13B 5′-ATT TTC CAC CAA CCC CCA GTT-3′
Lmj4 5′-CTA GTT TCC C GC CTC CGA G-3′ 94°C for 4 min, 94°C for 1 min, 55°C for 1 min, 72°C for 1 min, 72°C for 10 min (35 cycles) 1.5 0.2 1 1 2.5
Uni21 5′-GGG GTT GGT GTA AAA TAG GCC-3′
LITSR 5′-CTG GAT CAT TTT CCG ATG-3′ 94°C for 4 min, 94°C for 40 sec,53°C for 30 sec, 72°C for 1 min, 72°C for 10 min (40 cycles) 2 0.2 0.5 1.5 10
L5.8S 5′-TGA TAC CAC TTA TCG CAC TT 3′
GH20 5′-GAA GAG CCA AGG ACA GGT AC-3′ 94°C for 4 min, 94°C for 30 sec, 54.5°C for 1 min, 72°C for 1.3 min, 72°C for 10 min (40 cycles) 2.5 0.2 0.4 1 2
PCO4 5′-CAA CTT CAT CCA CGT TCA CC-3′