Molecular diagnosis of cutaneous leishmaniasis and identification of the causative Leishmania species in Morocco by using three PCR-based assays

Table 4 Results and performance of the five diagnostic assays for cutaneous leishmaniasis

Diagnostic method	Number of positives	True positives	Number of negatives	True negatives	Sensitivity (%)	Specificity (%)	PPV (%)	NPV (%)	χ ± [SE]
Direct examination	19	19	39	14	43	100	100	36	0.270 ± 0.110
Culture	13	13	45	14	29	100	100	31	0.170 ± 0.102
13A/13B PCR	48	44	10	10	100	71	92	100	0.791 ± 0.101
Lmj4/Uni21 PCR	35	35	23	14	79	100	100	61	0.652 ± 0.106
ITS1-PCR	45	44	13	13	100	93	98	100	0.950 ± 0.040
PCR c	44	44	14	14	100	100	100	100
Total		44		14

PPV: Positive Predictive Value; NPV: Negative Predictive Value; PCRc: PCR consensus results.
95% CI for sensitivity, specificity, PPV and NPV.

ISSN: 1756-3305