Phylogenetic analysis of the DAP-36 immunosuppressant proteins from hard ticks. Phylogram resulting from the alignment of 15 full-length protein sequences from the sialotranscriptomes of A. triste (At-17164, At-17166, At-9070, At-98384 and At-17165), A. parvum (Ap-16029, Ap-15676, Ap-10830, Ap-8059, Ap-12208 and Ap-20449) and A. cajennense (Ac-67048, Ac-58724, Ac-104824 and Ac-58982) as well as sequences from other hard ticks previously deposited in the NCBI protein database, including A. maculatum (Am-3467, Am-2059, Am-8781 and Am-0397), A. variegatum (Av-4090 and Av-3456), Ixodes ricinus (Ir-0457, Ir-8919, Ir-8705 and Ir-8701) and Dermacentor andersoni (Da-3605). The database access numbers used in this analysis are listed in Additional file 5: AF5. The neighbor-joining method with 1,000 bootstrap replicates was used for analysis in MEGA 5.05 software. Brackets highlight branches with more than 75% bootstrap support. The bar at bottom indicates 20% amino acid divergence.