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Figure 2 | Parasites & Vectors

Figure 2

From: DNA vaccination with a gene encoding Toxoplasma gondii Deoxyribose Phosphate Aldolase (TgDPA) induces partial protective immunity against lethal challenge in mice

Figure 2

Identification of TgDPA expression in E. coli BL21 (DE3) by SDS-PAGE and western-blotting. (A) Purified recombinant TgDPA protein was resolved by 12% SDS-PAGE gel and stained with coomassie brilliant blue R250. (B) Western blot of rTgDPA recombinant product probed with sera of rats experimentally immunized with rTgDPA. (C) Western blot of pVAX1/TgDPA expressed in BHK cells probed with anti-rTgDPA antibodies. (D) No band equivalent to TgDPA was observed in the negative control BHK cells transfected with the empty pVAX1 vector. (M) Represents Pre-stained protein marker.

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