Quantification of T lymphocytes and MHC molecules using flow cytometry analysis. Harvested at weeks 0, 2 and 4 (n = 5), splenocytes were used to quantify; (A) CD4+ T cells. (B): CD8+ T cells. (C): MHC-I molecules. (D) MHC-II molecules. Data were represented as means ± SD. The asterisk designates the significant differences (p < 0.05) between the groups. Results presented here were from three independent experiments.