Table 1 Sequences of primers used to amplify mitochondrial DNA regions from Chabertia erschowi and Chabertia ovina from China
Primer | Sequence (5′ to 3′) |
|---|---|
For rrn S | Â |
CHOF | TCGTTTAGTGGGTATGTGTGGTTCT (for C. ovina) |
CHOR | GCCTACTCCCTAACAAATGACGCTC (for C. ovina) |
CHEF | GTGGTTTTTAGGTTAGGGTTGAGTG (for C. erschowi) |
CHER | ACGCTCATACAAAGTAATAAACGCA (for C. erschowi) |
For nad 2 | Â |
CHOF | TTTGTGG(C\T)TAAGAGTGTT(G\A)GCTATT (for C. ovina) |
CHOR | GAGCCGTAATCAAACATAGTAAATC (for C. ovina) |
CHEF | TTTGTGG(C\T)TAAGAGTGTT(G\A)GCTATT (for C. erschowi) |
CHER | ACCGTAATCAAACATAGTAAAATCT (for C. erschowi) |
For C. ovina | Â |
COF | TGGTTGTGTGGTTTGGGCTCAT |
rrnLR | ATGTCCTCACGCTAAGACTGCC |
rrnLF | AGTTTGCTTCTGCCCAGTGA |
ND5R | ACCGTAACCTCGCCCATCCTG |
ND5F | ACGGCGTTAGTGGAGGAGGA |
ND1R | CCACTAACCAACTCCCTTTCACCC |
ND1F | ATTGGTGCTTTGCGGGCCAGT |
ND2R | CCATAAACCTTTAAAACCTCCC |
ND2F | TTGTTGGTTGGGAGACTATG |
CYR | AAAGGGTCCTCAACCAAACA |
CYF | CCTGTTTGGGGACCTTCTATTG |
COR | CCGCAGTAAAATAAGCACGAGA |
For C. erschowi | Â |
COF | ACCGACGGCTTATGGAAT |
rrnLR | AGTGCAACCCAACATTATACCCT |
rrnLF | TAAAGTTTGCTTCTGCCCAGTGATA |
ND1R | ATAATAGCCAACAAAAGCACCGACA |
ND1F | CTTGTCGGTGCTTTGCG |
CYR | CCGCCTCAATAAACATCTC |
CYF | TGGTCCAGATTATTGAAGG |
COR | TTACCCGTCAAATACAAAGT |