Efficacy of i.n. LaSP-Sol and LaSP-Ex against L. amazonensis infection. BALB/c mice received 20 μg of LaSP-Sol or LaSP-Ex by the i.n. route on days −14 and −7 of infection. Non-vaccinated controls received PBS alone. On day 0, animals were infected with 2 × 105 stationary-phase promastigotes of L. amazonensis-GFP (7 days of culture). A) Lesion sizes were measured at the indicated days and are expressed as the difference of thickness between non-infected and infected footpads. B) The parasite loads were measured on day 70 of infection and are expressed as fluorescence units (FU). The data displayed are representative of three independent experiments producing the same result profile. Means ± SD (n = 5). *p ≤ 0.05 and #p ≤ 0.01 in comparison to PBS controls.