LaSP-EX induces Th1 systemic polarization. Mice were vaccinated and infected as described for Figure 2. At day 7 of infection, their spleen cells were re-stimulated in vitro with LaAg (5 μg/mL), and after 48 hours of culture the levels of IFN-γ (A), IL-4 (B), IL-10 (C) and TGF-β (D) were measured in the supernatants. The data displayed is representative of three independent experiments. Means ± SD (n = 5). *p ≤ 0.05 in relation to PBS.